Antagonistic activity of three Aspergillus isolates against Fusarium wilt of banana

Iman Hidayat(1*), Lita Citra Dewi(2), Dalia Sukmawati(3)

(1) Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences – LIPI, Jln. Raya Jakarta-Bogor KM 46 Cibinong 16911 West Java, Indonesia
(2) Biology Department, Faculty of Mathematics and Natural Sciences, State University of Jakarta (UNJ), Jln. Pemuda No. 10, Rawamangun, Jakarta, Indonesia
(3) Biology Department, Faculty of Mathematics and Natural Sciences, State University of Jakarta (UNJ), Jln. Pemuda No. 10, Rawamangun, Jakarta, Indonesia
(*) Corresponding Author

Abstract


Endophytic fungi have been known to play a role in protecting their host plants against diseases and extreme environments. The purpose of this study was to determine the antagonistic activity of three Aspergillus isolates against F. oxysporum f. sp. cubense (Foc), and to identify the Aspergillus identity using multilocus phylogeny analysis. Antagonistic assay by dual culture method showed that the Aspergillus sp. strain PD2, strain PD4, and strain PD5 inhibited the growth of Foc isolate by 37.31%, 26.52%, and 12.04%, respectively. Multilocus phylogeny based on ITS rDNA, beta-tubulin, and calmodulin genes sequences showed that the Aspergillus strain PD2 and strain PD4 belong to A. section Terrei, while the Aspergillus sp. strain PD5 was identified as A. sydowii of the A. sect. Versicolores.


Keywords


biocontrol, endophyte, fungi, Fusarium wilt, multilocus phylogeny

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References


Altschul SF, Madden TL, Schaffer AA, Zhang, J, Zhang Z, Miller W, Lipman DJ. 1997. Gapped BLAST and psi-BLAST: A new generation of protein database search program. Nucleic Acids Res. 25, 3389-3402.

Barakat KM, Gohar YM. 2012. Antimicrobial agents produced by marine Aspergillus terreus var. africanus against some virulent fish pathogens. Indian J Microbiol 52(3), 366-372. DOI: 10.1007/s12088-012-0255-1

Chiu YL, Liaw SJ, Wu VC, Hsueh PR. 2005. Peritonitis caused by Aspergillus sydowii in a patient undergoing continuous ambulatory peritoneal dialysis. Journal of Infection 51(3), 159-161.

Cunningham CW. 1997. Can three incongruence tests predict when data should be combined? Mol. Biol. Evol. 14, 733-740.

Dennis C, Webster J. 1971. Antagonistic properties of species-groups of Trichoderma I. Production of non-volatile antibiotics. Trans. Br. Mycol. Soc. 57, 25-39.

Elmer WH. 2012. Cultural practices. pp. 67-74, in Gullino ML, Katan J, Garibaldi A (Eds.), Fusarium wilts of greenhouse vegetable and ornamental crops, APS Press.

Farag AM, Abd-Elnabey HM, Ibrahim HAH, El-Shenawy M. 2016. Purification, characterization and antimicrobial activity of chitinase from marine-derived Aspergillus terreus. The Egyptian Journal of Aquatic Research 42(2), 185-192.

Farris JD, Kallersjö M, Kluge AG, Bult C. 1995. Constructing a significance test for incongruence. Syst. Biol. 44, 570-572.

Fravel D, Olivain C, Alabouvette C. 2003. Fusarium oxysporum and its biocontrol. New Phytol. 157, 493-502.

Gao J, Weng H, Zhu D, Mingxue Y, Fangxia G, Yu X. 2008. Production and characterization of cellulolytic enzymes from the thermoacidophilic fungal Aspergillus terreus M11 under solid-state cultivation of corn stover. Bioresource technology 99, 7623-7629. DOI: 10.1016/j.biortech.2008.02.005.

Gebhardt K, Schimana J, Höltzel A, Dettner K, Draeger S, Beil W, Rheinheimer J, Fiedler HP. 2004. Aspochalamins A-D and Aspochalasin Z produced by the endosymbiotic fungus Aspergillus niveus LU 9575: I. Taxonomy, fermentation, isolation and biological activities. The Journal of antibiotics 57, 707-714. 10.7164/antibiotics.57.707.

Glass NL, Donaldson GC.1995. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl. Environ. Microbiol. 61, 1323-1330.

Gomathi S, Ambikapathy V. 2011. Antagonistic activity of fungi against Pythium debaryanum (Hesse) isolated from Chilli field soil. Advances in Applied Science Research 2 (4), 291-297.

Goutam J, Sharma G, Tiwari VK, Mishra A, Kharwar RN, Ramaraj V, Koch B. 2017. Isolation and characterization of “Terrein” an antimicrobial and antitumor compound from endophytic fungus Aspergillus terreus (JAS-2) associated from Achyranthus aspera Varanasi. India. Front. Microbiol. 8, 1334. doi: 10.3389/fmicb.2017.01334

Greco G, Capello M, Cecchi G, Cutroneo L, Di Piazza S, Zotti M. 2017. Another possible risk for the Mediterranean Sea? Aspergillus sydowii discovered in the Port of Genoa (Ligurian Sea, Italy). Marine Pollution Bulletin 122, 470-474. http://dx.doi.org/10.1016/j.marpolbul.2017.06.058

Hageskal G, Kristensen R, Fristad FR, Skaar I. 2011. Emerging pathogen Aspergillus calidoustus colonizes water distribution systems. Med. Mycol. 49, 588-593.

Heberle W, Loeffler W, König WA. 1974. Metabolic products of microorganisms. 136. Asposterol, an antibiotic from Aspergillus microcysticus. Arch Microbiol. 100(1), 73-95. (in germany language)

Hong SB, Go SJ, Shin HD, Frisvad JC, Samson RA. 2005. Polyphasic taxonomy of Aspergillus fumigatus and related species. Mycologia 97(6), 1316-1329.

Howard SJ, Harrison E, Bowyer P, Varga J, Denning DW. 2011. Cryptic species and azole resistance in the Aspergillus niger complex. Antimicrob Agents Chemother. 55(10), 4802-4809.

Kumar S, Stecher G, Tamura K. 2016. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33, 1870-1874.

Lee SK, Kang HG, Na KJ.Han JI. 2012. Fungal dermatitis caused by Aspergillus sydowii in a thoroughbred horse. Journal of Equine Veterinary Science 32, 835-839. DOI: 10.1016/j.jevs.2012.03.013

Liu X, Song F, Ma L, Chen C, Xiao X, Ren B, Liu X, Piggott AM, Av-Gay Y, Zhang L, Capon RJ, Dai H. 2013. Sydowiols A–C: Mycobacterium tuberculosis protein tyrosine phosphatase inhibitors from an East China Sea marine-derived fungus, Aspergillus sydowii. Tetrahedron Letters 54, 6081-6083. DOI: 10.1016/j.tetlet.2013.08.137

Liu N, Peng S, Yang J, Cong Z, Lin X, Liao S, Yang B, Zhou X, Zhou X, Liu Y, Wang J. 2019. Structurally diverse sesquiterpenoids and polyketides from a sponge associated fungus Aspergillus sydowii SCSIO41301. Fitoterapia 135, 27-32. DOI: 10.1016/j.fitote.2019.03.031

Molina AB, Williams RC, Hermanto C, Suwanda, Komolong B, Kokoa P. 2010. Final report: mitigating the threat of banana Fusarium wilt: understanding the agroecological distribution of pathogenic forms and developing disease management strategies. ABN 34 864 955 427. ACIAR, Canberra, Australia. pp. 1-76.

Nel B, Steinberg C, Labuschagne N, Viljoen A. 2007. Evaluation of fungicides and sterilants for potential application in the management of Fusarium wilt of banana. Crop Prot. 26, 697-705.

Peng HX, Sivasithamparama K, Turner DW. 1999. Chlamydospore germination and Fusarium wilt of banana plantlets in suppressive and conducive soils are affected by physical and chemical factors. Soil Biol. Biochem. 31, 1363-1374.

Ploetz RC. 2006. Fusarium wilt of banana is caused by several pathogens referred to as Fusarium oxysporum f. sp. cubense. Phytopathology 96, 653-656.

Ploetz RC. 2015a. Fusarium wilt of banana. Phytopathology 105, 1512-1521.

Ploetz RC. 2015b. Management of Fusarium wilt of banana: A review with special reference to tropical race 4. Crop Protection 73, 7-15.

Rajamanikyam M, Gade S, Vadlapudi V, Parvathaneni SP, Koude D, Dommati AK, Tiwari AK, Misra S, Sripadi P, Amanchy R. Upadhyayula SM. 2017. Biophysical and biochemical characterization of active secondary metabolites from Aspergillus allahabadii. Process Biochemistry 56, 45–56. DOI: 10.1016/j.procbio.2017.02.010

Sadorn K, Saepua S, Boonyuen N, Laksanacharoen P, Rachtawee P, Prabpai S, Kongsaeree P, Pittayakhajonwut P. 2016. Allahabadolactones A and B from the endophytic fungus, Aspergillus allahabadii BCC45335. Tetrahedron 72(4), 489-495. DOI: 10.1016/j.tet.2015.11.056.

Sethi BK, Jana A, Nanda PK, Das Mohapatra PK, Sahoo SL. 2016. Thermostable acidic protease production in Aspergillus terreus NCFT 4269.10 using chickling vetch peels, Journal of Taibah University for Science 10, 571-583. DOI: 10.1016/j.jtusci.2015.11.001

Simmonds NW. 1966. Bananas, 2nd ed. 512 pp. Longman, London.

Skidmore AM, Dickinson CH. 1976. Colony interactions and hyphal interference between Septoria nodorum and phylloplane fungi. Transactions of the British Mycological Society 66, 57-64.

Swofford DL. 2002. PAUP*: Phylogenetic analysis using parsimony (*and other methods), version 4. Sinauer Associates, Sunderland, Massachusetts.

Tiwari CK, Parihar J, Verma RK. 2011. Potential of Aspergillus niger and Trichoderma viride as biocontrol agents of wood decay fungi. J Indian Acad Wood Sci 8, 169-172. DOI: 10.1007/s13196-012-0027-x

Wang Y, Zheng J, Liu P, Wang W, Zhu W. 2011. Three new compounds from Aspergillus terreus PT06-2 grown in a high salt medium. Mar. Drugs 9, 1368-1378. DOI:10.3390/md9081368

Wang H, Zhao X, Liu C, Liu L, Yu S, Liang C, Guan T, Irfan M, Zhao K. 2014. Effects of the biocontrol agent Aspergillus flavipes on the soil microflora and soil enzymes in the rooting zone of pepper plants infected with Phytophthora capsici. Journal of Phytopathology 163, 513-521. DOI: 10.1111/jph.12347

White TJ, Bruns T, Lee S, Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In Innis MA, Gelfand DH, Sninsky JJ, White TJ (Eds.) PCR protocols-a guide to methods and applications. Acad. Pr. San Diego.

Xu L, Huang B, Wu Y, Huang Y, Dong T. 2011. The cost-benefit analysis for bananas diversity production in China Foc zones. Am. J. Plant Sci. 2, 561-568.

Yang SQ, Li XM, Li X. Chi LP, Wang BG. 2018. Two new diketomorpholine derivatives and a new highly conjugated ergostane-type steroid from the marine algal-derived endophytic fungus Aspergillus alabamensis EN-547. Mar. Drugs 16, 114. DOI:10.3390/md16040114

Zhuravleva OI, Afiyatullov SS, Denisenko VA, Ermakova SP, Slinkina NN, Dmitrenok PS, Kim NY. 2012. Secondary metabolites from a marine-derived fungus Aspergillus carneus Blochwitz. Phytochemistry 80, 123-131. DOI: 10.1016/j.phytochem.2012.05.008




DOI: https://doi.org/10.37604/jmsb.v1i1.16

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