Anti-oxidant and anti-bacterial activities of 11 Calophyllum species from Indonesia

Jamilah Abbas(1*)

(1) Research Centre for Chemistry. Indonesian Institute of Sciences, PUSPIPTEK Area, Serpong, Tangerang 15314, Indonesia
(*) Corresponding Author


Members of Calophyllum (Calophyllaceae) are commonly used as herbal medicine in Indonesia from the beginning of civilization. Due to their tremendous potential as a source for pharmaceutical industry, we selected 11 Calophyllum species for the current anti-oxidant and anti-bacterial studies, namely, Calophyllum euryphyllum, C. bicolor, C. flavoramulum, C incrassaptum, C. lowei, C. macrophyllum, C. nodosum, C. soulattri, C. tetrapterum, C. teysmannii, and C. tomentosum. The methods include Calophyllum stem bark extraction and separation into n-hexane, ethyl acetate, and butanol fractions, followed by the antioxidant analysis using DPPH (2,2-diphenyl-1-picrylhydrazyl radical scavenging) and the anti-bacterial activity against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Pseudomonas aeruginosa. The results showed that n-hexane fraction of C. soulattri exhibited the best anti-oxidant activity (IC50 = 2.2 µg/m). This study also showed that a xanthone compound isolated from C. macrophyllum, namely, 1,8 - dihydroxy-6 methoxy xanthone exhibited the anti-oxidant activity with IC50 = 1.56 ppm.


anti-bacterial, bioactive, Calophyllum, stem extract, xanthone

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