Determining the potential indigenous red-yeasts producing β-carotene and their phylogenetic relationship

I Nyoman Sumerta(1*), Yeni Yuliani(2), Atit Kanti(3)

(1) Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences (LIPI), Jalan Raya Jakarta-Bogor Km 46, Cibinong 16911, Indonesia
(2) Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences (LIPI), Jalan Raya Jakarta-Bogor Km 46, Cibinong 16911, Indonesia
(3) Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences (LIPI), Jalan Raya Jakarta-Bogor Km 46, Cibinong 16911, Indonesia
(*) Corresponding Author

Abstract


Red-yeasts are pigmented yeast species that could synthesize carotenoids for food supplements and pharmaceutical purposes. However, this group contains a lot of species which need to be explored thoroughly. The objective of this study was to view the β-carotene production of three indigenous red-yeasts by modifying glucose content in the growth medium and verifying their phylogenetic relationship. The glucose content modification in growth media influenced the β-carotene production of each species. Rhodosporidiobolus ruineniae (InaCCY1638/Y15Eg075) and Rhodosporidiobolus poonsokiae (InaCCY1606/Y15Kr068) produced higher β-carotene than Rhodotorula paludigena (InaCCY1527/Y15Eg004). These Rhodosporidiobolus species were able to produce higher β-carotene from 0.5 to 2% glucose content while Rhodotorula was low production in 2% glucose content. The higher producers by Rhodosporidiobolus species were clustered to Ruineniae clade and could be a potential clade for higher β-carotene production. Based on this result, using a high carotenoid producer of red-yeasts from indigenous strains is potential to be developed for β-carotene bioindustry in the future.

Keywords


β-carotene, glucose content, phylogenetic tree, red-yeasts

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DOI: https://doi.org/10.37604/jmsb.v1i2.31

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