Stability of Phycocyanin Extracted from Spirulina maxima in different pH from Indoor and Semi-outdoor Cultivation

Dwi Susilaningsih(1), Bimo Saskiaoktavian(2*), Ali Djunaedi(3), Agus Trianto(4)

(1) Research Center for Biotechnology, Indonesian Institute of Sciences, Jl. Raya Bogor KM 46, 16911 Cibinong, Bogor 2Depart
(2) Department of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University. Jl. Prof. Soedarto, SH. 50275 Tembalang, Semarang
(3) Department of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University. Jl. Prof. Soedarto, SH. 50275 Tembalang, Semarang
(4) Department of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University. Jl. Prof. Soedarto, SH. 50275 Tembalang, Semarang
(*) Corresponding Author

Abstract


Spirulina maxima is a blue-green microalga that rich in pigment. The pigments in S. maxima grouped into primary pigment (Chlorophyll a) and accessory pigments (carotenoid & phycobiliprotein). Phycocyanin is an accessory pigment that belongs to phycobiliprotein, blue colored, and can be used as natural food coloring and drugs. Phycocyanin has activities as antibacterial, antioxidant, anti-inflammatory, antihyperalgesic, and many more. Because of that, phycocyanin usually used in the pharmaceutical industry. However, phycocyanin is a protein that unstable under lights, high temperature, and pH in the storage. This study aims to obtain information about the effect of pH on the stability of phycocyanin extracted from S. maxima that cultivated in indoor and semi-outdoor. The steps are cultivation, extraction using different solutions to get blue pigment phycocyanin, and stability test. Phycocyanin was dissolved in a buffer solution at pH 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, and 7.0 for 7 days. Color observations based on visualization and concentration measurements are carried out every day to see changes in phycocyanin. Growth in indoor cultivation with 24 hours light is faster than semi-outdoor a that uses only sunlight. Cultivation gets the optical density value 0,6 at day 20 with indoor cultivation and day 34 with semi-outdoor cultivation. Results show that phycocyanin can be extracted using a buffer phosphate solution. The stability of the pigments can be seen from the color changes and relative concentration using a spectrophotometer. Phycocyanin shows stable in the storage of pH 4 until pH 5.5. The highest relative concentration (CR) was shown in pH 5.

Keywords


cultivation, microalga, phycocyanin, pigments, Spirulina maxima

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References


Antelo FS, Costa JAV, Kalil SJ. 2008. Thermal degradation kinetics of the phycocyanin from Spirulina Platensis. Biochemical Engineering Journal 41:43–47.

Arnold LE, Lofthouse N, Hurt E. 2012. Artificial food colors and attention-deficit/hyperactivity symptoms: conclusion to dye for. Neurotherapeutics 9:599–609.

Arylza IS. 2005. Phycocyanin from Microalgae Economic Value as an Industry Product. Oseana 30 (3) : 27 – 36.

Becker EW. 1994. Microalgae: Biotechnology and Microbiology. Cambridge University Press, Cambridge.

Berns DS, MacColl R. 1989. Phycocyanin in physical-chemical studies. Chem Rev 89:807–25.

Biggins J, Bruce D. 1989. Regulation of excitation energy transfer in organism containing phycobilins. Photosynth Res 20:1–34.

Bintang M. 2010. Biochemical: Research Techniques. Erlangga. Jakarta.

Chaiklahan R, Chirasuwan N, Bunnag B. 2012. Stability of phycocyanin extracted from Spirulina sp.: influence of temperature, pH and preservatives. Process Biochemistry.

Coyler CL, Kinkade CS, Viskari PJ, Landers JP. 2005. Analysis of cyanobacterial pigment and proteins by electrophoretic and chromatographic method. Anal Biochem Chem 382:559–569.

Dillon JC, Phuc AP, Dubacq JP. 1995. Nutritional value of the alga Spirulina. World Review of Nutrition and Dietetics 77: 32–46.

Dineshkumar R, Narendran R, Sampathkumar P. 2016. Cultivation of Spirulina platensis in Different Selective Media. Indian Journal of Geo Marine Sciences 45(12): 1749–1754.

Duangsee R, Phoopat N, Ningsanond S. 2009. Phycocyanin extraction from Spirulina platensis and extract stability under various pH and temperature. As J Food Ag-Ind 2(4): 819-826.

Glazer AN. 1989. The light guide: directional energy transfer in a photosynthetic antenna. J Biol Chem 264:1–4.

Hadiyanto H, Soetrisnanto D, Christwardhana M. 2014. Phytoremediation of palm oil mill effluent using Pistia Stratiotes plant and Algae Spirulina sp. for biomass production. International Journal of Engineering 27(12 (C)):1809–1814.

Hadiyanto, Suttrisnorhadi, Sutanto H, Suzery M, 2015. Phyocyanin extraction from microalgae Spirulina platensis assisted by ultrasound irradiation: effect of time and temperature. Songklanakarin J Sci Technol 38 (4):391-398.

Khan M I, Shin JH, Kim JD. 2018. The Promising future of microalgae: current status, challenges, and optimization of a sustainable and renewable industry for biofuels, feed, and other products. Microbial Cell Factories 17:36.

Imamoglu E, Sukan FV, Dalay MC. 2007. Effect of different culture media and light intensities on growth of Haematococcus pluvialis. J Natural Eng Sci 1 (3):05-09.

Jespersen L, Strømdahl LD, Olsen K, Skibsted LH. 2005. Heat and lightstability of natural blue colorants for use in confectionery and beverages. Eur Food Res Technol 220:261–266.

Kawaroe M, Pratomo T, Sunuddin A, Sari DW, Augustine D. 2009. Specific growth rate of Chlorella sp. and Dunaliella sp. according to different concentration of nutrient and photoperiod. Jurnal Ilmu-ilmu Perairan dan Perikanan Indonesia 16 (1) : 73-77.

Lavens P, Sorgeloos P. 1996. Manual on the production and use of live food for aquaculture. FAO Fisheries Technical Paper. Rome: Food and Agriculture Organization of the United Nations. 361.

Masojidek J, Koblizek M, Torzillo G. 2004. Photosynthesis in Microalgae in: A. Richmond (Ed). Handbook of Microalgal Culture: Biotechnology and Applied Phycology. Blakwell Science Ltd., Iowa. p.20-39.

Mishra SK, Shrivastav A, Mishra S. 2008. Effect of preservatives for food grade C-PC from Spirulina platensis. Process Biochem 43:339–45.

Nur MMA. 2014. Potency of microalgae as source of functional food in Indonesia (overview). Eksergi 11(2): 01-06.

Padyana AK, Bhat VB, Madyastha KM, Rajashankar KB, Ramakumar S. (2001). Crystal structure of light-harvesting protein C-phycocyanin from Spirulina platensis. Biochem Biophys Res Commun 282:893–898

Pelczar MJ, Chan ECS. 1988. Microbiology Volume 1. UI Press, Jakarta.

Raoof B, Kaushik BD, Prasanna R. 2006. Formulation of a Low Cost Medium for Mass Production of Spirulina. Biomass Bioenerg. 30: 537-42.

Richmond A. 1988. Open Systems for The Mass Production for The Photoautothropic Microalgae Outdoor: physiological principles. Journal of Application Phycology. 4:281-286.

Salisbury FB, Ross CW. 1995. Plant Physiology. ITB, Bandung.

Sarada R, Pillai MG, Ravishankar GA. 1999. Phycocyanin from Spirulina sp.: influence of processing of biomass on phycocyanin yield, analysis of efficiency of extraction methods and stability studies on phycocyanin. J Proc Biochem 34:795-801.

Sedjati S, Ridlo A, Supriyantini E. 2015. Effects of sugar addition on color stability of phycocyanin from Spirulina sp. Jurnal Kelautan Tropis 18 (1):1-6.

Stec B, Troxler RF, Teeter MM. 1999. Crystal structure of phycocyanin from Cyanidium caldarium provides a new perspective on phycobilisomes assembly. Biophys J 76:2912–2921.

Stevens L, Burgess J, Stochelski M, Kuczek T. 2013. Amounts of artificial food colors in commonly consumed beverages and potential behavioral implications for consumption in children: Revisited. Clinical Pediatrics 53.

Takano H, Arai T, Hirano M, Matsunaga T. 1995. Effects of intensity and quality of light on phycocyanin production by a marine Cyanobacterium Synechococcus sp. NKBG 042902. Applied Microbiology and Biotechnology 43(6): 1014–1018.

Wu H, Wang G, Xiang W, Li T,He H. 2016. Stability and antioxidant activity of food-grade phycocyanin isolated from Spirulina platensis. International Journal of Food Properties 19(10): 2349-2362, DOI: 10.1080/10942912.2015.1038564.




DOI: https://doi.org/10.37604/jmsb.v2i1.33

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