A molecular phylogenetic study of Clarohilum henningsii (Mycosphaerellaceae, Fungi) on cassava from Indonesia based on the ITS rDNA sequence

Iman Hidayat(1*), Aerma Hastuty(2), Indriati Ramadhani(3)

(1) Research Center for Biomaterials, Indonesian Institute of Sciences-LIPI, Jalan Raya Jakarta-Bogor Km 46, Cibinong 16911, Indonesia
(2) Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences, Cibinong
(3) (Scopus ID: 57195557566; SINTA ID: 6674426). Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences, Cibinong
(*) Corresponding Author

Abstract


Leaf spot is a common disease of cassava in Indonesia and other tropical countries. The present study aims to determine two isolates isolated from leaf spot of cassava in Indonesia using a molecular phylogenetic analysis based on internal transcribed spacer (ITS) rDNA sequence and morphological examination. The results showed that the two isolates belong to Clarohilum henningsii. This study revealed the phylogenetic position of C. henningsii from Indonesia, where in the previous studies, it has been reported as Cercospora cassavae, C. manihotis, C. henningsii, or Passalora henningsii based on morphological examinations.

Keywords


cassava, ITS rDNA, Indonesia, leaf spot, phylogenetic

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References


Bakhshi M, Zare R. 2020. Molecular confirmation of Nothopassalora personata causing leaf spot of peanut in Iran. Australasian Plant Disease Notes 15, 9. DOI: 10.1007/s13314-020-0380-3.

Chupp C. 1954. A Monograph of the Fungus Genus Cercospora. Ithaca, New York. Published by the author.

Edgar RC. 2004. Muscle: multiple sequence alligment with high accurancy and high throughput. Nucleic acids Res 32, 1792–1797. DOI:10.1093/nar/gkh340.

Farr DF, Rossman AY. 2020. Fungal Databases, U.S. National Fungus Collections, ARS, USDA. Retrieved June 11, 2020, from https://nt.ars-grin.gov/fungaldatabases/

Felsenstein J. 1985. Confidence limits on phylogenetic: an approach using the bootstrap. Evolution 39, 783–791.

Index Fungorum. 2020. Index Fungorum. Retrieved June 11, 2020, from http://www.indexfungorum.org/names/names.asp

Kumar S, Stecher G, Tamura K. 2016. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33, 1870-1874.

Saitou N, Nei M. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406-425.

Phengsintham P, Chukeatirote E, McKenzie EHC, Hyde KD, Braun U. 2013. Monograph of Cercosporoid fungi from Laos. Current Research in Environmental & Applied Mycology 3(1), 34– 158, DOI: 10.5943/cream/3/1/2.

Kimura M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16(2), 111-120.

van Overeem C. 1925. Cercosporaceae. Icones Fungorum Malayensium 10, 1–4.

Videira SIR, Groenewald JZ, Nakashima C, Braun U, Barreto RW, deWit PJGM, Crous PW. 2017. Mycosphaerellaceae – Chaos or clarity? Studies in Mycology 87, 257–421. DOI: 10.1016/ j.simyco.2017.09.003.

White TJ, Bruns T, Lee S, Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Pp. 315–322, in MA Innis et al. (eds.), PCR Protocols: a guide to methods and applications. San Diego, Academic Press.




DOI: https://doi.org/10.37604/jmsb.v2i1.43

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