Culturable bacterial abundance in Volvariella volvacea cultivation medium and characterization of its bacteria

Masrukhin Masrukhin(1*), Iwan Saskiawan(2)

(1) Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences-LIPI, Jalan Raya Jakarta-Bogor Km 46, Cibinong 16911, Indonesia
(2) Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences-LIPI, Jalan Raya Jakarta-Bogor Km 46, Cibinong 16911, Indonesia
(*) Corresponding Author

Abstract


Straw mushroom (Volvariella volvacea) is one of the popular edible fungi in Indonesia. Previous researches showed the correlation among the type of substrate, substrate quality, and its composting process to the microbial community, yield, and biological efficiency. The aim of the research is to analyze the culturable bacteria abundance in straw mushroom cultivation medium, characterize the bacteria in several stages of mushroom cultivation and investigate the interaction between V. volvacea with its resident bacteria. Samples were taken from mushroom farmers in Subang and Karawang regencies, Indonesia. The materials for cultivation medium are the mixture of cotton and paddy straw and the pasteurization was performed at 65-70°C for 7 hours. The result shows the abundance of the bacteria in most of the cultivation stages is relatively similar i.e. 108 CFU/g, except in 15 days after inoculation (DAI), the bacterial abundance is lower i.e.6.24 x 107 CFU/g. Twenty-five isolates were obtained and Gram-positive bacteria is the dominant bacteria found in the cultivation medium, especially rod-shaped Gram-positive bacteria. According to co-culture assay there are nine isolates that decrease the growth rate and clearly inhibit mycelial growth. The other 10 isolates have lower inhibitory activity, and 6 isolates have no inhibitory activity to the mycelial growth. C38 isolates have the highest mycelial growth inhibition. It belongs to rod-shaped Gram positive group of bacteria which isolated from the early stage of V. volvacea cultivation medium (5 DAI).

Keywords


bacterial diversity, bacterial abundance, Vorvariella volvacea

Full Text:

PDF

References


Carrasco J, Preston GM. 2020. Growing edible mushroom: a conversation between bacteria and fungi. Environmental Microbiology. DOI: 10.1111/1462-2920.14765.

Carrasco J, Zied DC, Pardo JE, Preston GM, Pardo-Gimenez A. 2018. Supplementation in mushroom crops and its impact on yield and quality. AMB Express 8:146. DOI: 10.1186/s13568-018-0678-0

Chen S, Qiu C, Huang T, Zhou W, Qi Y, Gao Y, Shen J, Qiu L. 2013. Effect of 1-aminocyclopropane -1- carboxylic acid deaminase producing bacteria on the hyphal growth and primordium initiation of Agaricus bisporus. Fungal Ecology 6:110-118. DOI: 10.1016/j.funeco.2012.08.003

Deveau A, Bonito G, Uehling J, Paoletti M, Becker M, Bindschedler S, et al. 2018. Bacterial-fungal interactions: ecology, mechanisms and challenges. FEMS Microbiol Rev. 42, 335–352. DOI: 10.1093/femsre/fuy008

Febriansyah E, Iwan S, Wibowo M. 2018. Potency of growth promoting bacteria on mycelial growth of edible mushroom Pleurotus ostreatus and its identification based on 16S rDNA analysis. AIP Conference Proceedings. DOI: 10.1063/1.5050119

Jemsi WS, Aryantha INP. 2017. Potential mushroom growth promoting bacteria (MGPB) in optimizing paddy straw mushroom production. Microbiology Indonesia 11(2): 46-54. DOI: 10.5454/mi.11.2.2

Kementerian Pertanian Indonesia [KEMENTAN]. 2019. Data produktivitas hortikultura periode 2016-2017. [Ministry of Agriculture of The Republic Indonesia. 2019. Horticultural productivity data for the period 2016-2017]. https://aplikasi2.pertanian.go.id. (accessed 31 May 2019).

Kertesz MA, Thai M. 2018. Compost bacteria and fungi that influence growth and development of Agaricus bisporus and other commercial mushrooms. Appl Microbiol Biotechnol. 102: 1639-1650. DOI: 10.1007/s00253-018-8777-z

Narayanasamy P. 2013. Biological Management of Diseases of Crops Vol. I: Characteristics of Biological Control Agents. Springer Science + Business Media.

Oh SY, Lim YW. 2018. Effect of fairy ring bacteria on the growth of Tricholoma matsutake in vitro culture. Mycorrhiza 28: 411-419. DOI: 10.1007/s00572-018-0828-x

Silva CF, Azevedo RS, Braga C, Silva RD, Dias ES, Schwan RF. 2009. Microbial diversity in a baggase-based compost prepared for the production of

Agaricus brasiliensis. Brazilian Journal of Microbiology 40: 590-600.

Vajna B, Nagy A, Sajben E. 2010. Microbial community structure changes during oyster mushroom substrate preparation. Appl Microbiol Biotechnol. 86(1): 367-375. DOI: 10.1007/s00253-009-2371-3

Xiang Q, Luo L, Liang Y, Chen Q, Zhang X, Gu Y. 2017. The diversity, growth promoting abilities and anti-microbial activities of bacteria isolated from the fruiting body of Agaricus bisporus. Polish Journal of Microbiology 66(2): 201-207.

Zarenejad F, Yakhchali B, Rasooli I. 2012. Evaluation of indigenous potent mushroom growth promoting bacteria (MGPB) on Agaricus bisporus production. World J Microbiol Biotechnol. 28(1): 99-104. DOI: 10.1007/s11274-011-0796-1

Zhang B, Yan L, Li Q, Zou J, Tan H, Tan W, Peng W, Li Z, Zhang X. 2018. Dynamic succession of substrate-associated bacterial composition and function during Ganoderma lucidum growth. Peer J 6: 1-23. DOI: 10.7717/peerj.4975

Zhang HL, Wei JK, Wang QH, Yang R, Ga XJ, Sang YX, Cai P, Zhang GQ, Chen QJ. 2019. Lignocellulose utilization and bacterial communities of millet straw-based mushroom (Agaricus bisporus) production. Scientific Reports 9(1): 1-13. DOI: 10.1038/s41598-018-37681-6

Zhang X, Zhong Y, Yang S, Zhang W, Xu M, Ma A, Zhuang G, Chen G, Liu W. 2014. Diversity and dynamics of the microbial community on decomposing wheat straw during mushroom compost production. Bioresource Technology 170:183-195. DOI:10.1016/j.biortech.2014.07.093

Zhu HX, Zuo Y, Qiu C, Wang X, Zhang Y, Shen J, Qiu L. 2013. Promotion of the growth and yield in Pleurotusostreatus by Bradyrhizobium japonicum. J Pure Appl Microbiol. 7:1087–1092.

Zikriyani H, Saskiawan I, Mangunwardoyo W. 2018. Utilization of agricultural waste for cultivation of paddy straw mushrooms (Volvariella volvacea (Bull.) Singer 1951). International Journal of Agricultural Technology 14(5): 805-814




DOI: https://doi.org/10.37604/jmsb.v2i2.52

Article Metrics

Abstract views : 134 | views : 40

Refbacks

  • There are currently no refbacks.




Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.


Copyright © 2019 by Indonesian Culture Collection (InaCC), Research Center for Biology
Indonesian Institute of Sciences (LIPI)

Cibinong Science Center (CSC), Jln. Raya Jakarta-Bogor KM. 46 Cibinong 16911, West Java, Indonesia
Telp. +62-21-8761356, Fax. +62-21-8761357
Available online: https://www.e-jmsb.id
E-mail: editor@e-jmsb.id
e-ISSN 2685-4430