Effect of ethanol extract from Karuk leaf (Piper sarmentosum Roxb.) on the growth of Malassezia furfur in vitro

Khusnul Khusnul(1*), Asti Kusmayanti(2), Lia Aulia Rahman(3), Nuniek I Ratnaningtyas(4)

(1) School of Technology Laboratory Medic, Sekolah Tinggi Ilmu Kesehatan Bakti Tunas Husada Tasikmalaya, Indonesia
(2) School of Technology Laboratory Medic, Sekolah Tinggi Ilmu Kesehatan Bakti Tunas Husada Tasikmalaya, Indonesia
(3) School of Technology Laboratory Medic, Sekolah Tinggi Ilmu Kesehatan Bakti Tunas Husada Tasikmalaya, Indonesia
(4) Faculty of Biology, Universitas Jenderal Soedirman
(*) Corresponding Author

Abstract


In Indonesia, there are numerous therapeutic plants found. Some of the plants used in herbal medicine are Karuk leaf (Piper sarmentosum Roxb.) belong to the Piperaceae family. Karuk leaf has chemical contents such as saponins, polyphenols, flavonoids, and essential oils and many tests are carried out on several bacteria, but testing of fungi is rarely studied. This study aims to determine the ethanol extract activities from karuk leaf in inhibiting the growth of the Malassezia furfur fungus and to determine its minimum inhibition by using the Kirby-Bauer method. The study was conducted by an experimental method of the M. furfur fungus using the Kirby-Bauer method. The ethanol extract from karuk leaf was made in various concentrations and tested on 0.5 McFarland fungus by diffusion on Sabouraud Dextrose Agar (SDA). The results of this analysis showed that the ethanol extract of Karuk leaf could inhibit the M. furfur fungus at a concentration of 30% by 5.3 mm, 40% by 6.6 mm, 50% by 7.6 mm, 60% by 11.3 mm, 70% by 12.5 mm, 80% by 15.6 mm, 90% by 17.4 mm, and 100% by 19.5 mm. Based on the results of the study and the data analysis, it can be concluded that several concentrations of ethanol extract of Karuk leaf affect the growth of M. furfur in vitro.

Keywords


effect, Karuk leaf, Kirby-Bauer, Malassezia furfur, mycosis

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References


Findley K, Oh J, Yang J et al. 2013. Topographic diversity of fungal and bacterial communities in human skin. Nature. 498: 367–370.

Franchimont CP, Pierard GE, Arrese JE, Doncker P. 2001. Effect of ketoconazole 1% and 2 % shampoos on severe dandruff and seborrhoeic dermatitis clinical, squamometric and mycological assesments. Dermathology., 202: 171-176.Gadjah Mada.

Gholib D. 2010. Uji daya hambat ekstrak etanol daun karuk (Piper sarmentosum ROXB.) dan daun seserehan (Piper aduncum L.) terhadap Trichophyton mentagrophytes. Prosiding Seminar Peternakan dan Veteriner. P. 815-819.

Khusnul, R Suhartati1 , DP Virgianti1, M Fathurohman1 , ATK Pratita1. 2019. Effect of Karuk Leaves (Piper sarmentosum Roxb) and White Galangal Rhizome (Alpinia galanga L) Ethanol Extract on the Growth of Microsporum Gypseum and Candida albicans in Vitro. IOP Conf. Series: Journal of Physics: Conf. Series 1179 (2019) 012168.

Khusnul, Deni W, Rudi H, dan Dewi P. V. 2017. Uji Efektivitas Ekstrak Etanol Rimpang Lengkuas (Alpinia galanga L) terhadap Pertumbuhan Trichophyton rubrum secara In Vitro. Jurnal Kesehatan Bakti Tunas Husada Tasikmalaya. Vol 17 (1) : 73-80

Nurhafani F. 2012. Perbandingan potensi antimikroba ekstrak n-heksana daun kelor (Moringa oleifera) dengan kulit biji (pericarp) jambu mete (Anacardium ocidentale) terhadap bakteri Pseudomonas aeruginosa. (Skripsi). Malang: Universitas Brawijaya.

Noer, I.S. dan L. Nurhayati. 2006. Bioaktivitas Ulva reticulate Forsskal. Asal Gili Kondo Lombok Timur terhadap Bakteri. Jurnal Botika. Vol. 5 (1) :45-60.

Oh J, Byrd AL, Deming C et al. 2014. Biogeography and individuality shape function in the human skin metagenome. Nature. 514: 59–64.

Shinta, 2002. Isolasi dan Identifikasi senyawa aktif antimikroba dari daun tumbuhan Piper sarmentosum roxb. Tesis Magister. Institute Tekhnologi Bandung.

Sutanto, Inge. Dkk. 2009. Parasitologi Kedokteran.Edisi Keempat, Balai Penerbit FKUI, Jakarta.

Steel, R.E.D., and J.H.Torrie. 1991. Prinsip dan Prosedur Statistika Suatu Pendekatan

Biometrik. Gramedia Pustaka Utama, Jakarta.

Theelen B.J.F, Claudia Cafarchia, Georgios Gaitanis, Ioannis Dimitrios Bassukas, Teun Boekhout and Thomas L. Dawson, Jr. 2018. Review Article Malassezia ecology, pathophysiology, and treatment. Medical Mycology, 2018, 56, S10–S25.

Virgianti, Rochmanah Suhartati, Resty Rosyani. 2017. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Karuk (Piper sarmentosum Roxb) terhadap Pertumbuhan Bakteri Streptococcus pyogenes. Jurnal Kesehatan Bakti Tunas Husada. Vol:17 (1). Hal :10-12.

Winarto, W.P, 2007. Tanaman Obat Indonesia. Untuk Pengobatan Herbal, jilid 3, Karya Sari Herba Media.




DOI: https://doi.org/10.37604/jmsb.v2i2.59

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