Extraction, characterization, and biological toxicity of β-glucans from Saccharomyces cerevisiae isolated from ragi

Indriati Ramadhani(1), Diva Larissa(2), Yeni Yuliani(3), Mellova Amir(4), Kusmiati Kusmiati(5*)

(1) Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences-LIPI, Jalan Raya Jakarta-Bogor Km 46, Cibinong 16911, Indonesia
(2) Institute Science and Technology National, Jl. Moh. Kahfi II, Jakarta Selatan 12640, Indonesia
(3) Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences-LIPI, Jalan Raya Jakarta-Bogor Km 46, Cibinong 16911, Indonesia
(4) Department of Pharmacy, Faculty of Health Science, Esa Unggul University, Jl. Arjuna Utara No.9 Kebon Jeruk, Jakarta 11510, Indonesia
(5) Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences-LIPI, Jalan Raya Jakarta-Bogor Km 46, Cibinong 16911, Indonesia
(*) Corresponding Author


β-glucan is a homopolysaccharide with biological activities that are beneficial to health as an immunostimulant, anti-inflammatory, anti-diabetic, anti-cholesterol, and many more. β-glucan extraction results from yeast require characterization related to this bioactive quality, such as β-glucan weight, monomer analysis, functional groups, and cytotoxicity assay. Four Saccharomyces cerevisiae isolates were isolated from three local ragi samples, namely the SC-1, SC-2, SC-3, and SAF from instant ragi. This study aimed to obtain the best candidate of S. cerevisiae isolates to produce high β-glucan levels and low protein levels and to test the potential for cytotoxicity. The four isolates were rejuvenated on potato dextrose agar (PDA), then inoculated into the liquid glucose yeast peptone (GYP) fermentation medium for six days. Saccharomyces cerevisiae cells were extracted by neutralizing acid-base, dried and weighed as a crude β-glucan (mg per 300 mL). The highest yield was SC-2 (818 mg), followed by SC-3 (726 mg), SAF (597 mg), and SC-1 (433 mg). The presence of –OH (alcohol), -C-C-C- (alkane), and –R-O-R- (ether) groups were showed using FTIR characterization. Glucose equivalent β-glucan levels and protein levels were determined using a UV-Vis spectrophotometer. The results showed that β-glucan SC-1 gave the best results with glucose equivalent β-glucan levels of 4,865% and protein levels of 3,804%. The crude β-glucan toxicity test using the brine shrimp lethality test (BSLT) method shows that the β-glucan of the SAF strain has LC50 cytotoxicity of 114.8 ppm followed by β-glucan cytotoxicity from local ragi LC50 was SC-2 (323.5 ppm), SC-1 (331.1 ppm), and SC-3 (354.8 ppm). Therefore, based on the results, SC-1 isolate obtained the highest β-glucan crude and the lowest protein content was SC-2. The β-glucan of SAF extract had the highest toxicity properties based on the IC50 value.


Brine Shrimp Lethality Test (BSLT), β-Glucans, FTIR, Glucose, Protein, S. cerevisiae

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Bzducha-Wróbel A, Błażejak S, Kieliszek M, Pobiega K, Falana K, Janowicz M. 2018. Modification of the cell wall structure of Saccharomyces cerevisiae strains during cultivation on waste potato juice water and glycerol towards biosynthesis of functional polysaccharides. Journal of biotechnology, 281, 1–10. DOI: 10.1016/j.jbiotec.2018.06.305

Curiel JA, Salvadó Z, Tronchoni J, Morales P, Rodrigues AJ, Quirós M, Gonzalez R. 2016. Identification of target genes to control acetate yield during aerobic fermentation with Saccharomyces cerevisiae. Microbial cell factories, 15(1), 156. DOI: 10.1186/s12934-016-0555-y

Di Luzio NR, Williams DL, McNamee RB, Edwards BF, Kitahama A. 1979. Comparative tumor‐inhibitory and anti‐bacterial activity of soluble and particulate glucan. International Journal of Cancer 24(6), 773–779. DOI: 10.1002/ijc.2910240613

Dongowski G, Huth M, Gebhardt E, Flamme W. 2002. Dietary fiber-rich barley products beneficially affect the intestinal tract of rats. The Journal of Nutrition 132(12), 3704–3714. DOI: 10.1093/jn/132.12.3704

Estrada A, Yun CH, Kessel AV, Li B, Hauta S, Laarveld B. 1997. Immunomodulatory activities of oat β-glucan in vitro and in vivo. Microbiology and Immunology 41(12), 991–998. DOI: 10.1111/j.1348-0421.1997.tb01959.x

Gonzaga MLC, Menezes TM, de Souza JRR, Ricardo NM, Soares SDA. 2013. Structural characterization of β-glucans isolated from Agaricus blazei Murill using NMR and FTIR spectroscopy. Bioactive carbohydrates and dietary fibre, 2(2), 152–156. DOI: 10.1016/j.bcdf.2013.10.005

Hallfrisch J, Scholfield DJ, Behall KM. 2003. Physiological responses of men and women to barley and oat extracts (Nu‐trimX). II. Comparison of glucose and insulin responses. Cereal Chemistry 80(1), 80–83. DOI: 10.1094/CCHEM.2003.80.1.80

Hunter Jr KW, Gault RA, Berner MD. 2002. Preparation of microparticulate β‐glucan from Saccharomyces cerevisiae for use in immune potentiation. Letters in Applied Microbiology 35(4), 267–271. DOI: 10.1046/j.1472-765X.2002.01201.x

Jenkins AL, Jenkins DJA, Zdravkovic U, Würsch P, Vuksan V. 2002. Depression of the glycemic index by high levels of β-glucan fiber in two functional foods tested in type 2 diabetes. European Journal of Clinical Nutrition 56(7), 622–628. DOI: 10.1038/sj.ejcn.1601367

Klis FM, Pieternella Mol, K. Hellingwerf, S. Brul. 2002. Dynamics of cell wall structure in Saccharomyces cerevisiae. FEMS Microbiology Reviews 26, 239–256. DOI: 10.1016/S0168-6445(02)00087-6

Kusmiati, Dhewantara, FX. 2016. Cholesterol-lowering effect of beta glucan extracted from Saccharomyces cerevisiae in rats. Scientia Pharmaceutica 84(1), 153–165. DOI: 10.3797/scipharm.ISP.2015.07

Lipke PN, Ovalle R. 1998. Cell wall architecture in yeast: new structure and new challenges. Journal of bacteriology 180(15), 3735–3740. DOI: 10.1128/JB.180.15.3735-3740.1998

Lotzová E, Gutterman JU. 1979. Effect of glucan on natural killer (NK) cells: further comparison between NK cell and bone marrow effector cell activities. The Journal of Immunology 123(2), 607–611.

Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DJ, McLaughlin JL. 1982. Brine shrimp: a convenient general bioassay for active plant constituents. Planta Medica 45(05), 31–34. DOI: 10.1055/s-2007-971236

Naseeb S, et al. 2017. Saccharomyces jurei sp. nov., isolation and genetic identification of a novel yeast species from Quercus robur. International journal of systematic and evolutionary microbiology, 67(6), 2046. DOI: 10.1099/ijsem.0.002013

Pengkumsri N, Sivamaruthi BS, Sirilun S, Peerajan S, Kesika P, Chaiyasut K, Chaiyasut C. 2017. Extraction of β-glucan from Saccharomyces cerevisiae: Comparison of different extraction methods and in vivo assessment of immunomodulatory effect in mice. Food Science and Technology, Campinas, 37(1), 124-130. DOI: 10.1590/1678-457X.10716

Ruiz-Herrera J, Ortiz-Castellanos L. 2019. Cell wall glucans of fungi. A review. The Cell Surface 5, 100022. DOI: 10.1016/j.tcsw.2019.100022

Sarah QS, Anny FC, Mir M. 2017. Brine shrimp lethality assay. Bangladesh Journal of Pharmacology 12(2), 186–189. DOI: 10.3329/bjp.v12i2.32796

Sofyan A, Herdian H, Sakti AA, Khairulli G, Jayanegara A. 2015. Physical and chemical properties of organic mineral additive for ruminant through biologically incorporated by Saccharomyces cerevisiae in difference substrates. Indonesian Journal of Applied Chemistry 17(2), 139–146. DOI: 10.14203/jkti.v17i2.30

Tungland BC. 2003. Fructooligosaccharides and other fructans: structures and occurrence, production, regulatory aspects, food applications, and nutritional health significance. ACS Publications. DOI: 10.1021/bk-2003-0849.ch011

Valle-Jones JC. 1985. An open study of oat bran meal biscuits ('Lejfibre') in the treatment of constipation in the elderly. Current Medical Research and Opinion 9(10), 716–720. DOI: 10.1185/03007998509109657

Wang J, Rosell CM, de Barber CB. 2002. Effect of the addition of different fibres on wheat dough performance and bread quality. Food Chemistry 79(2), 221–226. DOI: 10.1016/S0308-8146(02)00135-8

Williams DL, Pretus HA, McNamee RB, Jones EL, Ensley HE, Browder IW, Di Luzio NR. 1991. Development, physicochemical characterization and preclinical efficacy evaluation of a water soluble glucan sulfate derived from Saccharomyces cerevisiae. Immunopharmacology 22(3), 139–156. DOI: 10.1016/0162-3109(91)90039-2

DOI: https://doi.org/10.37604/jmsb.v2i2.62

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