Effect of Clove essential oil (Syzygium aromaticum) against the growth of dandruff scalps-causing fungal pathogen using Kirby- Bauer method in vitro

Khusnul Khusnul(1*), Puput Putuh Anisa(2), Dewi Peti Virgianti(3)

(1) School of Technology Laboratory Medic, Sekolah Tinggi Ilmu Kesehatan Bakti Tunas Husada Tasikmalaya, Indonesia
(2) School of Technology Laboratory Medic, Sekolah Tinggi Ilmu Kesehatan Bakti Tunas Husada Tasikmalaya, Indonesia
(3) School of Technology Laboratory Medic, Sekolah Tinggi Ilmu Kesehatan Bakti Tunas Husada Tasikmalaya, Indonesia
(*) Corresponding Author

Abstract


One of the strongest contributing factors in dandruff scalps is the presence of fungal pathogen or called as Malassezia. Several studies have found a connection between Malassezia and scalp health, one of which is caused by pathogenic fungal infections including Pityrosporum ovale, Microsporum gypseum, and Candida albicans. Clove flowers are known to contain eugenol and its derivative compounds which have antimicrobial, antifungal, antiseptic, and local anesthetic activity. This research aims to determine the effect of clove flower essential oil (Syzygium aromaticum) against some dandruff-causing fungi. The research method was to test the inhibitory power of clove flower essential oil using the diffusion method (Kirby Bauer technique). Samples of pathogenic fungi were cultured on SDA media (Saboroud Dextrose Agar) and given discs that had been given several concentrations of clove flower essential oil. The concentrations used were 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, positive control using 2% ketoconazole and negative control using virgin coconut oil (VCO). The results showed a significant effect between the ability of essential oils to inhibit the growth of some fungi that cause dandruff. The treatment of clove flower essential oil against M. gypseum at the concentration of 10% has an inhibitory ability of 33,05 mm and at the concentration of 100% amounted to 63.95 mm. Meanwhile, the inhibition percentage in P. ovale with a concentration of 10% and 100% of extract were 14,75 mm and 46,15 mm, respectively. The inhibition rate in C. albicans with the concentration of 10% extract and 100% of extract was 13,5 mm and 39,35 mm. The research shows that clove flower essential oil inhibits the growth of several pathogenic fungal in vitro.

Keywords


anti-dandruff, C. albicans, effect, Kirby-Bauer, mycosis, M. gypseum, P. ovale , Syzygium aromaticum,

Full Text:

PDF

References


Alfauziah TQ, & Budiman A. 2016. Uji aktivitas antifungi elmusi minyak atsiri bunga cengkeh terhadap Jamur Kayu : Jurnal Farmaka, 14 (1) : 37-40

Shinsetsu SA, Aditya MV, & Fauziyah S. 2015. Sintesis eugeno menjadi 2-metoksi-4-(1-propenil fenol melalui reaksi isomerisas dan aplikasinya sebagai baha suplemen pada mouthwash. Jurnal Sains dan Teknologi (Sainteknol), 3 (2).

Andries JR, Gunawan PN, & Supit A . 2014. Uji efek anti bakteri ekstrak bunga cengkeh terhadap bakteri Streptococcus mutans secara in vitro. Jurnal e-GiGi (eG), 2 (2).

Clinical and Laboratory standard Institute. Performance standards for antimicrobial disk susceptibility tests approved standard – Eleventh Edition, Pennsylvania, USA,2012

Febriyanti R, & Aldi BR. 2012. Pengaruh antifungi atsiri daun cengkeh (Syzygium aromaticum (L.) Merr. Perry). Parapemikir: Jurnal Ilmiah Farmasi, 1(2). DOI: 10.30591/pjif.v1i2.148

Khusnul, Hidana R, Kusmarini W. 2017. Uji efektifitas ekstrak etanol rimpang lengkuas (Alpiana Galanga L) terhadap pertumbuhan jamur Trichophyton rubrum secara invitro. STIKes Bakti Tunas Husada Tasikmalaya. Jurnal Kesehatan. 17:(1) 73-80.

Lova, IPST, Wijaya WA, Paramita NLPV, & Putra AARY. 2018. Perbandingan uji wataktivitas antibakteri minyak atsiri daun, tangkai bunga dan bunga cengkeh Bali (Syzygium aromaticum L.) terhadap bakteri Propionibacterium acne dengan metode difusi disk. Jurnal Kimia, 30. DOI: 10.24843/Jche m.2018.V12.I01.P06.

Moon S, Kim H, & Cha J. 2011. Synergistic effect between clove oil and its major compounds and antibiotics against oral bacteria. Archives of Oral Biology, 56(9): 907–916.

Nurhayati E, Kuswiyanto, Karta P. 2017. Pengaruh ekstrak bunga cengkeh (Syzygium Aromaticum) terhadap zona hambat jamur Tricophyton rubrum. Jurnal Laboratorium Khatulistiwa. 1 (1) : 26- 32.

Paliling A, Posangi J, Anindita PS. 2016. Uji daya hambat ekstrak bunga cengkeh terhadap bakteri Porphyromonas Gingivalis,Universitas SAM Ratulangi: Manado

Pandey A, & Singh P. 2011. Antibacterial activity of Syzygium aromaticum ( Clove ) with metal ion effect against food borne pathogens. 1(2), 69–80.

Pathirana HNKS, Wimalasena SHMP, Desilva BCJ, Hossain S, & Gang-Joon H. 2019. Antibacterial activity of clove essential oil and eugenol against fish pathogenic bacteria isolated from cultured olive flounder (Paralichthys olivaceus). Slovenian Veterinary Research, 56(1): 31–38. DOI: 10.26873/SVR- 590-2018.

Pratiwi Sylvia. T. 2008. Mikrobiologi Farmasi. Erlangga : Jakarta.

Prianto H, et al. 2013. Isolasi dan karakteristik dari minyak bunga cengkeh (syzgium aromaticum) kering hasil distilasi uap. Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Brawijaya : Kimia Student Journal. 1(2) : 269-274

Rahmadani. 2012. The effect of lime utilization on healing dry dandruff on the scalp. Faculty of Engineering, State University of Padang.

Saikumari D, Rani SKS, & Saxena N. 2016. Antibacterial activity of Syzigium aromaticum L. (Clove). International Journal of Current Microbiology and Applied Sciences, 5(11): 484–489. DOI:10.20546/Ijcmas.2 016.511.056

Sundari DM, Winarno W. 2001. Informasi tumbuhan obat sebagai anti jamur. Depkes RI. Jakarta Gandahusada, Sriasi. 2003. FKUI, Jakarta

Towaha J, 2012. Manfaat eugenol cengkeh pada berbagai industri di Indonesia. Balai Penelitian Tanaman Industri dan Penyegar : Perspetif. 11 (2) : 105-107

Utami RT, Dewi SS, Darmawati S. 2019. Antibacterial activity of clove stem extract (syzigium aromaticum) against bacterial growth methicillin-resisten Staphylococcus aureus (MRSA). Proceeding of Unimus. 2.

Yuliana A. 2014. Uji aktibitas antijamur formulasi elmusi minyak cengkeh (Syzgium aromaticum L. Merr). Jurnal Kesehatan Bakti Tunas Husada . 11 (1) : 26-57




DOI: https://doi.org/10.37604/jmsb.v3i1.78

Article Metrics

Abstract views : 150 | views : 62

Refbacks

  • There are currently no refbacks.




Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.


Copyright © 2019 by Indonesian Culture Collection (InaCC), Research Center for Biology
Indonesian Institute of Sciences (LIPI)

Cibinong Science Center (CSC), Jln. Raya Jakarta-Bogor KM. 46 Cibinong 16911, West Java, Indonesia
Telp. +62-21-8761356, Fax. +62-21-8761357
Available online: https://www.e-jmsb.id
E-mail: editor@e-jmsb.id
e-ISSN 2685-4430