Biosynthesis and profiling of single cell carotenoids of Phaf ia rhodozyma in waste-based cultivation media

Arif Nurkanto(1*), Rini Handayani(2), Ismu Purnaningsih(3), Mia Kusmiati(4), Ninu Setianingrum(5), Mulyadi Mulyadi(6), Endang Kusdiyanti(7), Achmad Dinoto(8)

(1) Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences Jl. Raya Jakarta-Bogor km. 46, Cibinong Science Center, Cibinong 16911 Indonesia
(2) Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences Jl. Raya Jakarta-Bogor km. 46, Cibinong Science Center, Cibinong 16911 Indonesia
(3) Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences Jl. Raya Jakarta-Bogor km. 46, Cibinong Science Center, Cibinong 16911 Indonesia
(4) Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences Jl. Raya Jakarta-Bogor km. 46, Cibinong Science Center, Cibinong 16911 Indonesia
(5) Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences Jl. Raya Jakarta-Bogor km. 46, Cibinong Science Center, Cibinong 16911 Indonesia
(6) Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences Jl. Raya Jakarta-Bogor km. 46, Cibinong Science Center, Cibinong 16911 Indonesia
(7) Departement Biology, Faculty of Science and Mathematic, Diponegoro University, Jl. Prof. Soedarto, SH. Tembalang, Semarang, Central Java, Indonesia
(8) Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences Jl. Raya Jakarta-Bogor km. 46, Cibinong Science Center, Cibinong 16911 Indonesia
(*) Corresponding Author

Abstract


In order to investigate the feasibility of low-cost media for producing well- characterized single-cell carotenoid, the study on biosynthesis and profiling carotenoid in the yeast of Phaf ia rhodozyma was carried out. We have successfully identified the profiles of single-cell carotenoids of P. rhodozyma, which was cultivated in glucose-based medium (MG), molasses-based medium (MM), and coconut water-based medium (MC). Cells were separately cultured in those media under aerobic batch culture system to obtain the carotenoid profiles based on high performance liquid chromatography (HPLC) analysis. The results showed that medium composition strongly affects the profiles of P. rhodozyma carotenoids represented by ratio of astaxanthin and beta-carotene (ratio A/B). Astaxanthin was highly synthesized in cells cultivated in MG with ratio A/B as much of 20:1. On the other hand, MM and MC produced a lower ratio A/B than MG as much of 0.4:1 and 0.2:1, respectively. In addition, carotenoids profiles were detected more diverse when this yeast species was cultivated in two waste-based media. This study provided a basic physiological knowledge of P. rhodozyma cells for carotenoid biosynthesis using potential low cost cultivation m

Keywords


single cell carotenoid, Phaffia rhodozyma, waste-based medium, beta-carotene, astaxanthin

Full Text:

PDF

References


Armstrong GA. 1997. Genetics of eubacterial carotenoid biosynthesis: a colorful tale. Annual Reviews in Microbiology 51 (1): 629-659.

Barreiro C, Barredo JL. 2018. Carotenoids production: A healthy and profitable industry. Methods in Molecular Biology. DOI: 10.1007/978-1-4939-8742-9_2.

Bogacz-Radomska L, Harasym J. 2018. β-Carotene-properties and production methods, Food Quality and Safety. DOI: 10.1093/fqsafe/fyy004.

Braunwald T, Schwemmlein L, Graeff-Hönninger S, Todd FW, Hernandez R, Holmes WE, Claupein W. 2013. Effect of different C/N ratios on carotenoid and lipid production by Rhodotorula glutinis. Appl. Microbiol Biotechnol 97:6581-6588.

Fang TJ, Cheng YS. 1993. Improvement of astaxanthin production by Phaf ia rhodozyma through mutation and optimization of culture conditions. Journal of Fermentation and Bioengineering 75 (6): 466-469.

Hui N, Qi-he C, Hui R, Yuan-fan Y, Li-jun L, Guang-bin W, Yang H, Guo-qing H. 2007. Studies on optimization of nitrogen sources for astaxanthin production by Phaf ia rhodozyma. Journal of Zhejiang University SCIENCE B 8 (5): 365-370.

Johnson EA. 2003. Phaf ia rhodozyma: colorful odyssey. International Journal of Microbiology 6 (3): 169-174. Krinsky NI. 1993. Actions of carotenoids in biological systems. Annual review of nutrition 13 (1): 561-587.

Lee P, Schmidt-Dannert C. 2002. Metabolic engineering towards biotechnological production of carotenoids in microorganisms. Applied Microbiology and Biotechnology 60: 1

Lewis MJ, Ragot N, Berlant MC, Miranda M. 1990. Selection of astaxanthin-overproducing mutants of Phaf ia rhodozyma with  -Ionone. Applied and Environmental Microbiology 56: 2944-2945.

Lodato P, Alcaino J, Barahona S, Retamales P, Jimenez A, Cifuentes V. 2004. Study of the expression of carotenoid biosynthesis genes in wild-type and deregulated strains of Xanthophyllomyces dendrorhous (Ex.: Phaf ia rhodozyma). Biological Research 37 (1): 83-93.

Mata-Gómez LC, Montañez JC, Méndez-Zavala A, Aguilar C N. 2014. Biotechnological production of carotenoids by yeasts: An overview. In Microbial Cell Factories. DOI: 10.1186/1475-2859-13-12

Miller NJ, Sampson J, Candeias LP, Bramley PM, Rice-Evans CA. 1996. Antioxidant activities of carotenes and xanthophylls. FEBS Letters 384 (3): 240-242.

Nelis H, De Leenheer AP. 1991. Microbial sources of carotenoid pigments used in foods and feeds. Journal of Applied Bacteriology 70 (3): 181-191. Prades A, Dornier M, Diop N, Pain JP. 2012. Coconut water uses, composition and properties: A review. In Fruits. DOI: 10.1051/fruits/2012002

Ramıŕ ez J, Gutierrez H, Gschaedler A. 2001. Optimization of astaxanthin production by Phaffia rhodozyma through factorial design and response surface methodology. Journal of Biotechnology 88 (3): 259-268.

Schroeder WA, Johnson EA. 1993. Antioxidant role of carotenoids in Phaffia rhodozyma. Journal of General Microbiology 139 (5): 907-912.

Sinnot, R. 1988. Fish pigmentation. Trout News 7:8-11.

Sulistyo J, Handayani R, Soeka,YS. 2016. Biosynthesis and antioxidant activity of single cell carotenoids extracted from microbial cultures. Journal of Innovations in Pharmaceuticals and Biological Sciences, JIPBS, Vol 3 (1), 133-142.

Sumerta IN, Yuliani Y, Kanti, A. 2019. Determining the potential indigenous red-yeasts producing β-carotene and their phylogenetic relationship. Journal of Microbial Systematics and Biotechnology. DOI: 10.37604/jmsb.v1i2.31

Sun N, Lee S, Song KB. 2004. Characterization of a carotenoid-hyperproducing yeast mutant isolated by low-dose gamma irradiation. International Journal of Food Microbiology 94 (3): 263-267.

Yamane Y, Higashida K, Nakashimada Y, Kakizono T and Nishio N. 1997. Influence of oxygen and glucose on primary metabolism and astaxanthin production by Phaf ia rhodozyma in batch and fed-batch cultures: Kinetic and stoichiometric analysis. Applied and Environmental Microbiology 63 (11): 4471-4478




DOI: https://doi.org/10.37604/jmsb.v3i1.61

Article Metrics

Abstract views : 97 | views : 43

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Journal of Microbial Systematics and Biotechnology Indexed By:


Copyright © 2019 by Indonesian Culture Collection (InaCC), Research Center for Biology
Indonesian Institute of Sciences (LIPI)
Cibinong Science Center (CSC), Jln. Raya Jakarta-Bogor KM. 46 Cibinong 16911, West Java, Indonesia
Telp. +62-21-8761356, Fax. +62-21-8761357
Available online: https://www.e-jmsb.id
E-mail: editor@e-jmsb.id
e-ISSN 2685-4430