Biosynthesis and profiling of single cell carotenoids of Phaf ia rhodozyma in waste-based cultivation media

Arif Nurkanto(1*), Rini Handayani(2), Ismu Purnaningsih(3), Mia Kusmiati(4), Ninu Setianingrum(5), Mulyadi Mulyadi(6), Endang Kusdiyanti(7), Achmad Dinoto(8)

(1) Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences Jl. Raya Jakarta-Bogor km. 46, Cibinong Science Center, Cibinong 16911 Indonesia
(2) Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences Jl. Raya Jakarta-Bogor km. 46, Cibinong Science Center, Cibinong 16911 Indonesia
(3) Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences Jl. Raya Jakarta-Bogor km. 46, Cibinong Science Center, Cibinong 16911 Indonesia
(4) Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences Jl. Raya Jakarta-Bogor km. 46, Cibinong Science Center, Cibinong 16911 Indonesia
(5) Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences Jl. Raya Jakarta-Bogor km. 46, Cibinong Science Center, Cibinong 16911 Indonesia
(6) Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences Jl. Raya Jakarta-Bogor km. 46, Cibinong Science Center, Cibinong 16911 Indonesia
(7) Departement Biology, Faculty of Science and Mathematic, Diponegoro University, Jl. Prof. Soedarto, SH. Tembalang, Semarang, Central Java, Indonesia
(8) Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences Jl. Raya Jakarta-Bogor km. 46, Cibinong Science Center, Cibinong 16911 Indonesia
(*) Corresponding Author

Abstract


In order to investigate the feasibility of low-cost media for producing well- characterized single-cell carotenoid, the study on biosynthesis and profiling carotenoid in the yeast of Phaf ia rhodozyma was carried out. We have successfully identified the profiles of single-cell carotenoids of P. rhodozyma, which was cultivated in glucose-based medium (MG), molasses-based medium (MM), and coconut water-based medium (MC). Cells were separately cultured in those media under aerobic batch culture system to obtain the carotenoid profiles based on high performance liquid chromatography (HPLC) analysis. The results showed that medium composition strongly affects the profiles of P. rhodozyma carotenoids represented by ratio of astaxanthin and beta-carotene (ratio A/B). Astaxanthin was highly synthesized in cells cultivated in MG with ratio A/B as much of 20:1. On the other hand, MM and MC produced a lower ratio A/B than MG as much of 0.4:1 and 0.2:1, respectively. In addition, carotenoids profiles were detected more diverse when this yeast species was cultivated in two waste-based media. This study provided a basic physiological knowledge of P. rhodozyma cells for carotenoid biosynthesis using potential low cost cultivation m

Keywords


single cell carotenoid, Phaffia rhodozyma, waste-based medium, beta-carotene, astaxanthin

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DOI: https://doi.org/10.37604/jmsb.v3i1.61

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